The Biochemistry of the Nucleic Acids by Roger L. P. Adams, John T. Knowler, David P. Leader (auth.)

By Roger L. P. Adams, John T. Knowler, David P. Leader (auth.)

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A) In wheatgerm DNA guanine and cytosine are not present in equimolar amounts but this is explained by the scarcity of cytosine being compensated for by the presence of 5-methylcytosine. (b) In 4>X174 DNA and in the DNA of several similar small coliphages the adenine is not equimolar with thymine nor is guanine with cytosine. This is because 4>X174 DNA is single-stranded. 4). Conventional analytical equilibrium ultracentrifugation is unsatisfactory since the available instruments are not stable at speeds low enough to balance centrifugal forces against diffusion forces.

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