The Biochemistry of Inorganic Polyphosphates by Igor S. Kulaev, Vladimir Vagabov, Tatiana Kulakovskaya

By Igor S. Kulaev, Vladimir Vagabov, Tatiana Kulakovskaya

Now in a moment variation, Biochemistry of Inorganic Polyphosphates fills the necessity for an exhaustive source on inorganic polyphosphate metabolism. The authors describe the constitution and houses of those compounds and offers a comparative research of the most recent and conventional equipment in their extraction from cells. Distribution of polyphosphates in organisms, their localization in cells and tissues is additionally defined. * accomplished presentation of inorganic polyphosphate metabolism * Follows polyphosphates in cells of organisms from assorted phases of evolution * provides tools for the research and learn of polyP-dependent enzymes * complete details on genetics, metabolism and biotechnology of polyphosphates * Textbook and reference paintings on all facets of polyphosphates

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9. 8. It can be seen that the contribution of the middle (n c ) groups to the total intensity of the P peaks decreases with an increase in the PolyP chain length. 2) In the above equation, SPc is the total intensity (peak area) of signals from the middle phosphate groups, SPst is the standard Pi signal intensity, and C is the PolyP concentration, expressed in mg Pi per ml. The effect of reduction of the contribution of inner phosphate groups to the total intensity of the Pc signals is obviously associated with the primary and secondary structures of PolyPs.

1989). The intensities of the signals in the study of PolyPs by using 31 P NMR spectroscopy directly depend on the degree of PolyP binding with other structures and compounds of Gel electrophoresis 31 the cell, in particular, with metal ions. , 1986). , 1996). The interaction with cations lays the basis for the 31 P NMR spectroscopic method, allowing one to distinguish between extracellular and intracellular pools of PolyPs. Ethylenediaminetetraacetic acid (EDTA) can be used to complex the divalent cations bound to PolyPs and to produce a new 31 P NMR shift.

2 Separation of PolyPs of low molecular weight by ion-exchange chromatography on Dowex 1 XI0 in a KCl gradient. The numbers 1–12 represent the number of phosphorus atoms in the PolyP molecules constituting the various fractions (Matsuhashi, 1963). high-molecular-weight PolyPs. The first of these is Van Wazer’s method for the fractional precipitation of PolyPs of different chain lengths from aqueous solutions with acetone (Van Wazer, 1958). This technique yields a great number of fractions, which differ from each other in the lengths of the PolyP chains.

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