Software Engineering and Knowledge Engineering: Theory and by Lingxia Liu (auth.), Yanwen Wu (eds.)

By Lingxia Liu (auth.), Yanwen Wu (eds.)

The quantity encompasses a set of chosen papers prolonged and revised from the I2009 Pacific-Asia convention on wisdom Engineering and software program Engineering (KESE 2009) was once hung on December 19~ 20, 2009, Shenzhen, China.

Volume 2 is to supply a discussion board for researchers, educators, engineers, and govt officers focused on the final components of data Engineering and conversation know-how to disseminate their most up-to-date study effects and trade perspectives at the destiny examine instructions of those fields. a hundred thirty five top quality papers are incorporated within the quantity. every one paper has been peer-reviewed by means of no less than 2 software committee participants and chosen by way of the quantity editor Prof.Yanwen Wu.

On behalf of the this quantity, we wish to specific our honest appreciation to all of authors and referees for his or her efforts reviewing the papers. Hoping you will find plenty of profound learn rules and effects at the comparable fields of information Engineering and conversation expertise.

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Extra info for Software Engineering and Knowledge Engineering: Theory and Practice: Volume 2

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PDN19LacΩ, Gluconacetobacter xylinum was first discovered the bacteria biosynthesized bacterial cellulose (BC) and used extensively up to now [1,2]. BC was widely applied in fields of material and medical, chemical, food and bio-engineering because of high production and purity. So the genetic operations of G. xylinum were studied in a deep-going way and the wild strains were constructed by gene engineering. All of these were set up to obtain the new strains of higher-yield or better BC [3]. The electroporation technique has the advantages of simple, rapid and high transformation frequency and was applied from the end of 1980s.

8kV), respectively. 22 H. Zhu et al. Fig. 2. 7 Identification of Transformant Control plates were not colonies genetation. As shown in Figure 3a, transformant was cultured and biosynthesized BC membrane in LB liquid medium contained spectinomycin 10 μg /ml and streptomycin 10 μg /ml while wild type strain was not growth. Plasmid pDN19LacΩ digested by Hind III was divided into two fragments with 6 kb and 2 kb respectively. Several transformants were randomly picked from screening plate and the plasmid DNA was abstracted from transformants.

Y. ): Software Engineering and Knowledge Engineering: Vol. 2, AISC 115, pp. 17–24. com 18 H. Zhu et al. transforming, so it was often used in test with charging high transforming efficiency [4,5,6,7]. The electroporation techniques of G. xylinum have not made a systematical and profound study for the present. In addition, plenty of BC biosynthesized in process of culture lead to competent problems of G. xylinum. In this study, the enzymolysis condition for removing bacterial cellulose of G. xylinum was optimized by Response Surface Method; optimum transmission conditions including growth stage of the strain, electroshock voltage, concentration and preservation of competent cell were defined for the electroporation with plasmid pDN19LacΩ.

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