Satellites and Defective Viral RNAs by K. A. White, T. J. Morris (auth.), Professor Peter K. Vogt

By K. A. White, T. J. Morris (auth.), Professor Peter K. Vogt Ph.D., Andrew O. Jackson Ph.D. (eds.)

The 9 chapters provided during this booklet offer modern stories of analysis on faulty RNAs, satellite tv for pc RNA viruses, and established RNA viruses that require the presence of a helper virus with a purpose to identify efficient infections. due to the fact their preliminary id approximately 4 many years in the past, primary analyses of pathogenic and interdependent interactions related to those brokers have contributed significantly to our appreciation of virus constitution, RNA replication, and techniques resulting in sickness. Findings coming up from those reports have additionally complicated a variety of ancillary parts, together with constitution and serve as of nucleic acids and proteins, nucleoprotein interactions, translational mechan­ isms, RNA processing, macromolecular evolution, and a plethora of different forte themes. learn on those subviral pathogens is constant to light up numerous facets of biology, chemistry, and biotechnology, so the publication is meant to supply a present therapy that may be worthwhile for readers with pursuits in issues on the topic of those parts. Observations within the early I 960s first published faulty virus, satellite tv for pc tobacco necrosis virus (STNV), is linked to and depends upon the presence of a hel in step with virus, tobacco necrosis virus (TNV), for its multiplication. This discovering ushered in a brand new period in virology that quickly led to a extra profound apprecia­ tion of the relationships of viruses and their interactions with every one other.

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Additional info for Satellites and Defective Viral RNAs

Example text

A large percentage 01' reported satellite RNA sequences correspond to different variants present in a single field isolate of CMV, or generated upon pas- 40 F. Garcia-Arenal and P. Palukaitis sage under controlled conditions (examples are listed in Fig. land references therein). Reported sequences for the linear, single-stranded, CMV satellite RNA molecules fall into two size dasses: Most of them are of 332-342 nucleotide residues; satellite RNAs in this size dass have been found in CMV isolates from all over the world and from many different host plant species and families.

_aaCCCCUGGGAGGc __ . L-. Fig. 2A,B. Sat-RNA C promoter sequences. A Hairpin promoter located at thc 3' end 01' sat-RNA C plus strands. ArrOll' denotes the transcription initiation site. The similar sequence at thc 3' end 01' TCV genomic RNA is also shown. B Thc two promoters located on sat-RNA C minus strands. 1'proximal promoter is hoxed and is the only 3' end sequcncc required for transcription in vitro. ArrOir denotes the wild-type transcription initiation site. In the ahsence 01' the 3'-tenninal six hases (CCCUA U), or if nontemplate nucleotides are added to the 3' end 01' the minus strand.

WL2. WL3). (BI. B2_ B3). (X2c. Xln. X15. SqlO). (D-CARNA5. n-CARNA5). (Y. , ToOW3l2 wtl >0< ... ß& 42 F. Garcia-Aremd and P. 012).

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