Handbook of Microbiological Media, Fourth Edition by Ronald M. Atlas

By Ronald M. Atlas

Handbook of Microbiological Media, Fourth Edition is a useful reference for each clinical, veterinary, diagnostic, and educational laboratory, and now in its fourth variation, it really is much more whole. This variation consists of at the culture of CRC Press guide excellence, directory the formulations, tools of coaching, and makes use of for greater than 7,000 microbiological media. With 1,500 extra entries than any past variation, the guide comprises either vintage and glossy media used for the identity, cultivation, and upkeep of numerous micro organism, archaea, and fungi.

The breadth of tradition media during this entire source is big and has significantly extended in recent times with the exploration of maximum habitats and using molecular the right way to establish new lineages of micro organism and archaea. The media additionally symbolize major advances within the skill to exploit chromogenic substrates to spot particular species and lines of micro organism, e.g., E. coli O157 and methicillin-resistant Staphylococcus aureus (MRSA). those media are super worthy for scientific diagnostics and for the security of the meals provide from pathogenic microorganisms.

With over 7,000 formulations, this re-creation contains all of the media used for:

  • Routine exam of meals and water
  • Cultivating particular lines of micro organism, archaea, fungi, and protists, together with many anaerobes and extremophiles
  • Cultivating the various microorganisms at the moment to be had from the world’s worldwide bioresource facilities (BRCs)

Additionally, many tradition media at the moment are to be had which are freed from animal elements. Plant-based media cast off attainable infection with prions, that is vital for creation of vaccines and pharmaceuticals.

The entries are prepared alphabetically by way of medium identify and comprise composition, directions for instruction, advertisement resources, protection cautions, makes use of, and extra. This reference includes the main complete compilation of microbiological media to be had in one quantity. the single source you wish for all media varieties, it makes discovering media for culturing varied microorganisms fast and straightforward. With uniform displays of media formulations and arrangements, it provides easy-to-follow instructions and cookbook recipes for getting ready media. You won’t discover a extra entire or basic microbiology reference anywhere.

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Example text

0g CaCO3 ......................................................................................... 0g Agar ............................................................................................ 0g Yeast extract................................................................................ 0L. Mix thoroughly to evenly distribute CaCO3. 5. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool rapidly to 50°–55°C. Pour into sterile Petri dishes or leave in tubes.

Preparation of Medium: Add components, except vitamin solution, Agar ............................................................................................ 0g CaCO3 ......................................................................................... 0g Yeast extract................................................................................ 0g Glucose ......................................................................................... 0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

8–7 by gassing. Gently heat and bring to boiling. Continue boiling for 3 min. Cool to room temperature while sparging with 80% N2 + 20% CO2. 7mL volumes into anaerobic tubes. Autoclave for 20 min at 15 psi pressure–121°C. 1mL of sterile Na2S·9H2O solution to each tube. Mix thoroughly. Use: For the cultivation of Acetitomaculum ruminus. 0mL: Cellobiose or cellulose (MN 300, Whatman CF II, Kleenex tissue paper, or HCl-treated cotton)............................................................. 0g NaHCO3 ........................................................................................

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