Epstein Barr Virus: Methods and Protocols by Janos Minarovits, Hans Helmut Niller

By Janos Minarovits, Hans Helmut Niller

This quantity explores facts from the functions of molecular organic tools and the functions of modern immunological and cytogenetic equipment in Epstein-Barr Virus (EBV) that may provide readers attainable new strategies to the unresolved difficulties within the EBV box. Chapters during this booklet conceal issues reminiscent of: viral lifestyles cycle, latency, EBV-associated illnesses and EBV diagnostics; in vitro tools together with organotypic cultures for the research of EBV-epithelial mobilephone interactions; id of the interacting viral and mobile proteins utilizing affinity purification-mass spectrometry equipment; 3D telomere FISH; transcription research utilizing high-throughput RNA sequencing, qPCR and nuclear run-on assay; research of viral and mobile microRNAs; isolation and characterization of exosomes and the evaluation in their functionality; characterization of the viral genome by means of terminal repeat research and sequencing; using chromatin immunoprecipitation coupled sequencing (ChIP-Seq) for the research of Zta-DNA interactions; epigenetic research by means of bisulfite sequencing and ChIP; novel in vivo versions for the learn of EBV an infection; and the way immunological, virological, tissue tradition and molecular equipment should be mixed to yield strong production Practice-compliant EBV-specific T cells for the immunotherapy of EBV-associated post-transplant lymphoproliferative illnesses (PTLD). Written within the hugely profitable Methods in Molecular Biology series structure, chapters comprise introductions to their respective issues, lists of the mandatory fabrics and reagents, step by step, effortlessly reproducible laboratory protocols, and tips about troubleshooting and heading off identified pitfalls.

Cutting-edge and entire, Epstein Barr-Virus: equipment and Protocols is a beneficial source for a person who's attracted to this attention-grabbing and evolving field.

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Boccellato F, Anastasiadou E, Rosato P, Kempkes B, Frati L, Faggioni A, Trivedi P (2007) EBNA2 interferes with the germinal center phenotype by downregulating BCL6 and TCL1 in non-Hodgkin’s lymphoma cells. J Virol 81(5):2274–2282. 01822-06 101. Roughan JE, Thorley-Lawson DA (2009) The intersection of Epstein-Barr virus with the germinal center. J Virol 83(8):3968– 3976. 02609-08 102. Roughan JE, Torgbor C, Thorley-Lawson DA (2010) Germinal center B cells latently infected with Epstein-Barr virus proliferate extensively but do not increase in number.

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