Antiviral Methods and Protocols by Derek Kinchington, Raymond F. Schinazi

By Derek Kinchington, Raymond F. Schinazi

Royal London tuition of drugs, united kingdom. moment writer, Raymond F. Schinazi, is at Emory Univ., Decatur, GA. bargains simple and scientific researchers types to guage compounds powerful opposed to acute and persistent infections. contains finished assays, caliber trying out, and unpublished equipment. DNLM: Hepatitis, Viral, Human - drug treatment.

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8. O’Brien, S. , and Shannon, J. E. (1977) Enzyme polymorphisms as genetic signatures in human cell cultures. Science 195, 1345–1348. 9. Jeffreys, A. , and Thein, S. L. (1985) Individual-specific ‘fingerprints’ of human DNA. Nature 316, 76–79. 10. Stacey, G. , Bolton, B. , and Doyle A. (1992) DNA fingerprinting transforms the art of cell authentication. Nature 357, 261,262. 11. Stacey, G. , Bolton, B. , Clark, S. , and Doyle, A. (1992) Multilocus DNA fingerprint analysis of cell banks: Stability and culture identification in human B lymphoblastoid and mammalian cell stocks.

2. Remove solutions by inverting microtiter plate and then tapping on a clean dry paper towel. 25 mL of blocking solution into all antibody coated wells and incubate at 37°C for 30 min. 3. Remove solutions as above. 25 mL washing solution buffer and finally remove washing buffer thoroughly. 4. 5 mL sample buffer. 25 mL of sample buffer to either 1 mL of either sterile media analogs to the sample or 1 mL of washing buffer. 5. 2 mL of prepared sample into the eight designated sample wells. 2 mL of negative and positive control (as supplied) into each of the designated wells.

25. 26. 27. 28. 29. RPMI-1640 (without L-glutamine). L-Glutamine. Fetal bovine serum (FBS). Trypsin-versine solution. HEPES-buffered saline (HBS). G418 (Genticin™, Gibco-BRL, Gaithersburg, MD). 3TC (lamivudine, (–)β-L-2',3'-dideoxy-3'-thiacytidine). Dimethyl sulfoxide (DMSO) (tissue culture grade). Formaldehyde (37%, Sigma-Aldrich, Milwaukee, WI). Neutral red dye. Acetic acid/EtOH (1% glacial acetic acid [GAA]/50% EtOH in H2O). Dulbecco’s phosphate-buffered saline (DPBS) with calcium and magnesium.

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